SpectraMax M Multifunksjonell plateleser

SpectraMax M2 / M2e

General Specifiations
Dimensions (in.)	8.6 (H) x 22.8 (W) x 15 (D)
Dimensions (cm)	22 (H) x 58 (W) x 38 (D)
Weight	35 lbs. (15.9 kg)
Power consumption	< 125 watts
Power source	100-240 Vac, 3 A, 50/60 Hz
Absorbance Photometric Performance
Wavelength range	200-1000 nm
Wavelength selection	Monochromator, tunable in 1.0 nm increments
Wavelength bandwidth	≤ 4.0 nm
Wavelength accuracy	±2.0 nm
Wavelength repeatability	±0.2 nm
Photometric range	0-4.0 OD
Photometric resolution	0.001 OD
Photometric accuracy (microplate)	< ±0.006 OD ±1.0%, 0-2 OD
Photometric accuracy (cuvette)	< ±0.005 OD ±1.0%, 0-2 OD
Photometric precision	< ±0.003 OD ±1.0%, 0-2 OD
Baseline flatness	< 0.001 OD
Stray light	< 0.05% @ 230 nm
Fluorescence Photometric Performance (M2)
Dual monochromators	1 nm increments
EX	250-850 nm
EM	250-850 nm
Bandwidth (EX, EM)	9, 9 nm
Optimized sensitivity (top-read)	5 pM (fluorescein)
Guaranteed sensitivity (top-read)**	15 pM (fluorescein)
Fluorescence Photometric Performance (M2e)
Dual monochromators	1 nm increments
EX	250-850 nm
EM	360-850 nm
Bandwidth (EX, EM)	9, 9 nm
Optimized sensitivity (bottom-read M2e only)	10 pM (fluorescein)
Sensitivity (bottom-read)**	25 pM (fluorescein)
Top-read detection limit	3.0 fmol/well FITC 200 μL in 96 wells (signal 3X SD of baseline)
Bottom-read detection limit	5.0 fmol/well FITC 200 μL in 96 wells (signal 3X SD of baseline)
Time-Resolved Fluorescence (Secondary Mode)
Wavelength range (M2)	360-850 nm
Wavelength range (M2e)	250-850 nm
Data collection	50-1450 μsec., 200 μsec. increments
Sensitivity**	7 pM europium
Luminescence (Secondary Mode)
Wavelength range (M2)	360-850 nm
Wavelength range (M2e)	250-850 nm
Sensitivity**	50 fM alkaline phosphatase
General Photometric Performance
Plate formats	6, 12, 24, 48, 96, 384 wells
Light source	Xenon flash lamp (1 joule/flash)
Detector	Photomultiplier (R-3896)
Read time* 96-well	Abs 18 sec., FI 15 sec.; 384-well: Abs 49 sec., FI 45 sec.
Shaker time	0 to 999 seconds
Temp. control	4°C above ambient to 45°C
Temp. uniformity	< 1°C at 37°C set point
Temp. accuracy	±1°C at 37°C set point
* Measurement type may extend read time.

 

SpectraMax M3

General Specifications
Dimensions (in.)				8.6 (H) x 22.8 (W) x 15.3 (D)
Dimensions (cm)				22 (H) x 58 (W) x 39 (D)
Weight				36 lbs (16.4 kg)
Power Consumption				< 420 watts
Power Source				100-240 VAC, 3 A, 50/60 Hz
Robotic Compatible				Yes
General Photometric Performance
Plate Formats				6, 12, 24, 48, 96, 384 wells
Light Source				Xenon Flash Lamp (1 joule/flash)
Detectors				2 photomultiplier tubes
ShakerTime				0 to 999 seconds
Temperature Control				2 °C above ambient to 60 °C
Temperature Uniformity				< 1°C at 37°C set point
Temperature Accuracy				±1°C at 37°C set point
Endpoint Reading				All modes
Kinetic Reading				All modes
Spectral Scanning				All modes
Well Scanning				Abs, FI, Lum
Standard Read Times (minutes:seconds)††
				96 wells			384 wells
Absorbance				0:18			0:49
Fluorescence Intensity				0:17			0:48
Luminescence				2:00			7:00
Absorbance Photometric Performance
Reading Capabilities				Cuvette or microplate
Wavelength				200-1000 nm
Wavelength Selection				Monochromator, tunable in 1.0 nm increments
Wavelength Bandwidth				< 4.0 nm
Wavelength Accuracy				±2.0 nm
Wavelength Repeatability				±0.2 nm
Photometric Range				0-4.0 OD
Photometric Resolution				0.001 OD
Photometric Accuracy (Microplate)				< ±0.006 OD ±1.0%, 0-2 OD
Photometric Accuracy (Cuvette)				< ±0.005 OD ±1.0%, 0-2 OD
Photometric Precision				< ±0.003 OD ±1.0%, 0-2 OD
Stray Light				< 0.05% @ 230 nm
Fluorescence Intensity Performance
Reading Capabilities				Cuvette or top or bottom of a microplate
Wavelength Range				250-850 nm
Wavelength Selection				Monochromators, tunable in 1.0 nm increments
Bandwidth (Ex, Em)				9 nm, 15 nm
Optimized sensitivity				≤ 1 pM fluorescein in 96 wells or cuvette < 1.5 pM in 384 wells
Sensitivity**				< 5 pM fluorescein in 96 wells < 20 pM in 384 wells
Luminescence Performance
Reading Capabilities				Cuvette or top or bottom of a microplate
Wavelength Selection				All wavelengths or with selected wavelengths
Wavelength Range				250-850 nm
Optimized sensitivity				≤ 43 pM ATP in 96 wells
Sensitivity**				≤ 75 pM ATP in 96 wells
Cross-talk				< 0.3% in 96- and 384-well microplates

 

SpectraMax M4

General Specifications
Dimensions (in.)				8.6 (H) x 22.8 (W) x 15.3 (D)
Dimensions (cm)				22 (H) x 58 (W) x 39 (D)
Weight				36 lbs (16.4 kg)
Power Consumption				< 420 watts
Power Source				100-240 VAC, 3 A, 50/60 Hz
Robotic Compatible				Yes
General Photometric Performance
Plate Formats				6, 12, 24, 48, 96, 384 wells
Light Source				Xenon Flash Lamp (1 joule/flash)
Detectors				2 photomultiplier tubes
ShakerTime				0 to 999 seconds
Temperature Control				2 °C above ambient to 60 °C
Temperature Uniformity				< 1°C at 37°C set point
Temperature Accuracy				±1°C at 37°C set point
Endpoint Reading				All modes
Kinetic Reading				All modes
Spectral Scanning				All modes
Well Scanning				Abs, FI, TRF, Lum
Standard Read Times (minutes:seconds)††
				96 wells	384 wells
Absorbance				0:18	0:49
Fluorescence Intensity				0:17	0:48
Time-Resolved Fluorescence				0:17	0:48
Luminescence				2:00	7:00
Absorbance Photometric Performance
Reading Capabilities				Cuvette or microplate
Wavelength Range				200-1000 nm
Wavelength Selection				Monochromator, tunable in 1.0 nm increments
Wavelength Bandwidth				< 4.0 nm
Wavelength Accuracy				±2.0 nm
Wavelength Repeatability				±0.2 nm
Photometric Range				0-4.0 OD
Photometric Resolution				0.001 OD
Photometric Accuracy (Microplate)				< ±0.006 OD ±1.0%, 0-2 OD
Photometric Accuracy (Cuvette)				< ±0.005 OD ±1.0%, 0-2 OD
Photometric Precision				< ±0.003 OD ±1.0%, 0-2 OD
Stray Light				< 0.05% @ 230 nm
Fluorescence Intensity Performance
Reading Capabilities				Cuvette or top or bottom of a microplate
Wavelength Range				250-850 nm
Wavelength Selection				Monochromators, tunable in 1.0 nm increments
Bandwidth (Ex, Em)				9 nm, 15 nm
Optimized sensitivity				≤ 1 pM fluorescein in 96 wells ≤ 1.5 pM in 384 wells
Sensitivity**				< 5 pM fluorescein in 96 wells or cuvette ≤ 20 pM in 384 wells
Luminescence Performance
Reading Capabilities				Cuvette or top or bottom of a microplate
Wavelength Selection				All wavelengths or with selected wavelengths
Wavelength Range				250-850 nm
Optimized sensitivity				≤43 pM ATP in 96 wells
Sensitivity**				≤75 pM ATP in 96 wells
Cross-talk				< 0.3% in 96- and 384-well microplate
Time-Resolved Fluorescence Performance
Reading Capabilities				Top or bottom of a microplate
Wavelength Range				250-850 nm
Wavelength Selection				Monochromators, tunable in 1.0 nm increments
Bandwidth (Ex, Em)				9 nm, 15 nm
Precision Data Collectionn				1-100 flashes, delay of 0-600 µsec before read, integration time selectable between 50-1500 µsec
Optimized sensitivity				≤10 fM europium in 96 wells
Sensitivity**				≤100 fM europium in 96 or 384 wells
SpectraMax M5e reader only				Certified to Cisbio Bioassays' HTRF assays performance Specifications

 

SpectraMax M5 / M5e

General Specifications
Dimensions (in.)	8.6 H x 22.8 W x 15.3 D
Dimensions (cm)	22 H x 58 W 39 D
Weight	35-36 lbs (15.9-16.4kg)
Power Consumption	< 125 Watts
Power Source	100-240 VAC, 3 A, 50/60 Hz
Robotic Compatible	Yes
Generic Photometric Performance
Plate Formats	6, 12, 24, 48, 96, 384 wells
Light Source	Xenon Flash Lamp (1 joule/flash)
Detectors	1-2 photomultiplier tubes
ShakerTime	0 to 999 seconds
Temperature Control	2-4 °C above ambient to 45-60 °C
Temperature Uniformity	< 1°C at 37°C set point
Temperature Accuracy	±1°C at 37°C set point
Endpoint Reading	All modes
Kinetic Reading	All modes
Spectral Scanning	All modes
Well Scanning	Abs, FI, TRF, Lum
Standard Read Times (minutes:seconds)††
	96 wells	384 wells
Absorbance	0:18	0:49
Fluorescence Intensity	0:17	0:48
Fluorescence Polarization	0:42	2:03
Time-Resolved Fluorescence	0:17	0:48
Luminescence	2:00	7:00
Absorbance Photometric Performance
Reading Capabilities	Cuvette or microplate
Wavelength Range	200-1000nm
Wavelength Selection	Monochromator, tunable in 1.0 nm increments
Wavelength Bandwidth	< 4.0nm
Wavelength Accuracy	±2.0 nm
Wavelength Repeatability	±0.2 nm
Photometric Range	0-4.0 OD
Photometric Resolution	0.001 OD
Photometric Accuracy (Microplate)	< ±0.006 OD ±1.0%, 0-2 OD
Photometric Accuracy (Cuvette)	< ±0.005 OD ±1.0%, 0-2 OD
Photometric Precision	< ±0.003 OD ±1.0%, 0-2 OD
Stray Light	< 0.05% @ 230 nm
Fluorescence Intensity Performance
Reading Capabilities	Cuvette or top or bottom of a microplate
Wavelength Range	250-850 nm
Wavelength Selection	Monochromators, tunable to 1.0 nm increments
Bandwidth (Ex, Em)	9nm, 15 nm
Optimized sensitivity	≤ 1 pM fluorescein in 96 wells ≤ 1.5 pM in 384 wells
Sensitivity**	< 5 pM fluorescein in 96 wells or cuvette < 20 pM in 384 wells
Luminescence Performance
Reading Capabilities	Cuvette or top or bottom of a microplate
Wavelength Selection	All wavelengths or with selected wavelengths
Wavelength Range	250-850 nm
Optimized sensitivity	≤ 43 pM ATP in 96 wells
Sensitivity**	≤ 75 pM ATP in 96 wells
Cross-talk	< 0.3% in 96- and 384-well microplates
Time Resolved Fluorescence Performance
Reading Capabilities	Top or bottom of a microplate
Wavelength Range	250-850nm
Wavelength Selection	Monochromators, tunable in 1.0 nm increments
Bandwidth (Ex, Em)	9nm, 15 nm
Precision Data Collection	1-100 flashes, delay of 0-600µsec before read, integration time selectable between 50-1500 µsec
Optimized sensitivity**	≤ 10 fM europium in 96 wells
Sensitivity**	≤ 100 fM europium in 96 or 384 wells
SpectraMax M5e reader only	Certified to Cisbio Bioassays' HTRF assays performance Specifications
Fluorescence Polarization Performance
Wavelength Range	300-750 nm
Wavelength Selection	Monochromators, tunable in 1.0 nm increments
Bandwidth (Ex, Em)	9 nm, 15 nm
Precision**	≤ 3.5 mP standard deviation at 1 nM fluorescein in 96 wells