HiBiT technology in protein analyses
Detect and quantify any tagged protein with a simple, luminescent signal
New HiBiT technology brings the power of bioluminescence to protein analysis.
HiBiT simplifies protein tagging in live cells, providing a streamlined, antibody-free protocol for detecting tagged proteins that requires only a luminometer for detection. With the dynamic range to detect proteins in live cells without overexpression, and the convenience of a single-reagent-addition method, HiBiT technology opens up a universe of possibilities for researchers studying protein biology.
Applications of HiBiT Technology
Tag Endogenous Proteins via CRISPR Insertion—No Cloning Required
HiBiT technology makes CRISPR–mediated tag knock-ins accessible by eliminating the need for molecular cloning prior to insertion and simplifying detection of tagged proteins. The small tag size makes CRISPR insertion efficient and the bioluminescence enables antibody-free detection with the sensitivity to detect endogenous expression.
Lytic method
Sensitive quantification of HiBiT-tagged proteins in cell lysates, IP complexes, or other cell-free systems.
Extracellular method
Used with live cells to quantify surface-expressed or secreted proteins.
Blotting system
Detects any tagged protein on a blot in a few minutes with a simple luminescent signal.