Fast Luminescent Detection of Proteins on Blots
- Determine protein size and quantify expression on blots
- Protocol requires only minutes, with few processing steps
- Femtogram sensitivity proportional over five orders of magnitude
Rapid Detection of HiBiT-Tagged Proteins on Blots
The Nano-Glo® HiBiT Blotting System visualizes HiBiT-tagged proteins on blots at subpicogram levels. The reaction uses a detection reagent containing LgBiT Protein, which complements the HiBiT tag to form the luminescent NanoBiT® enzyme.
The blotting system requires as little as 5 minutes to detect HiBiT-tagged proteins on a nitrocellulose membrane. Standard antibody-based blotting protocols can take multiple hours to detect the protein of interest.