The GenePrint 10 System allows co-amplification and three-color detection of nine human loci, including the ASN-0002 loci (TH01, TPOX, vWA, Amelogenin, CSF1PO, D16S539, D7S820, D13S317 and D5S818) as well as D21S11. These loci collectively provide a genetic profile with a random match probability of 1 in 2.92 x 10(9). The GenePrint 10 System is compatible with the ABI PRISM 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. You may need to optimize protocols including the amount of template DNA, cycle number, injection conditions and loading volume for your laboratory instrumentation. The GenePrint 10 System contains all materials necessary to amplify STR regions of human genomic DNA, including a hot-start thermostable DNA polymerase, which is a component of the GenePrint 10 5X Master Mix. An internal lane standard (ILS) and allelic ladder are provided for standardization, and the 2800M Control DNA is supplied as a positive control. The ILS is added to every sample after amplification and used within each capillary electrophoresis run to determine the size of each amplified product. The allelic ladder consists of the most common alleles at a particular locus and is used as a standard to positively identify each allele. GenePrint 10 Allelic Ladder Mix information, including the size range and repeat numbers for each allele, can be found in the Technical Manual. The 2800M Control DNA has a known genotype and can be used to verify genotyping accuracy.|
Amplification of ANSI-0002-Recommended Loci (plus Amelogenin and D21S11 for extra power of discrimination): Accurately discriminate between biological samples and human cell lines. The resulting STR profiles are compatible with publicly available databases. Fewer loci simplify data interpretation. Improved Buffer Formulation: Compatibility with direct amplification from FTA(R), and nonFTA cards saves labor and time and reduces manipulation and possible introduction of inhibitors or contaminants. Tolerance of Higher DNA Template Input: Better balance for aneuploid samples. Reduced PCR Time: Amplify in less than 1.5 hours. One Complete Kit: Validated and quality-control tested for sample identification and cell line authentication. Automatic Assignment of Genotypes: Panels and bins text files are required to automatically assign genotypes using the GeneMapper(R), ID and ID-X software and are available for download.