The small size (19.1kDa) and extreme brightness (about 100-fold brighter than either firefly [Photinus pyralis] or Renilla reniformis) of NanoLuc luciferase (Nluc) make it an ideal protein fusion partner. NanoLuc fusion proteins can be used in a variety of applications including: reporters of protein stability, probes for bioluminescent cell imaging (BLI) or as the donor signal in bioluminescent resonance energy transfer (BRET) applications for protein:protein or protein:small-molecule interaction studies. The NanoLuc protein fusion vectors enable simple generation of N- or C-terminal fusions of NanoLuc luciferase with your protein of interest and are available in two formats to accommodate your cloning preferences: 1)pNLF Vector series: Generate N- or C-terminal fusions to the full-length Nluc protein or attach secreted Nluc to the N-terminus of the protein of interest using traditional cloning with a multiple cloning site (MCS). 2)pF Vector series: Generate N- or C-terminal Nluc fusion proteins using the Flexi Vector Cloning System—a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, that provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence.|
Easily Quantify Changes in Protein Abundance: Use the single-addition Nano-Glo(R), Luciferase Assay System to quantify the signal from NanoLuc(R), fusion proteins to measure intracellular protein levels.
Obtain Improved Biological Relevance: Bright NanoLuc(R), reporter enables endogenous expression levels of NanoLuc(R), fusion proteins to avoid overexpression artifacts.
Visualize Intracellular Protein Dynamics: Bright NanoLuc(R), reporter enables reduced imaging exposure times without the need for repeated sample excitation, which can result in cytotoxic artifacts.
Improve BRET Studies: The brighter signal and blue-shifted emission spectrum from NanoLuc(R), luciferase result in less spectral overlap with fluorescent acceptors, resulting in better signal:background and dynamic range for BRET applications.
Flexible Cloning Options: Easily attach NanoLuc(R), luciferase to the N- or C-terminus of your protein of interest using either traditional or Flexi(R), cloning systems.
Easily Transition from Transient to Stable Cells: All vectors contain a mammalian selectable marker to create a stable line.