The PowerQuant System is a 5-color, 4-target probe-based qPCR assay that simultaneously quantifies the total amount of amplifiable autosomal and Y-chromosomal DNA in a single assay using the same DNA standards. During amplification, annealing of the probe to its target sequence generates a substrate that is cleaved by the 5' nuclease activity of Taq DNA polymerase when the enzyme extends from an upstream primer into the region of the probe. This liberates the fluorescent dye from its proximal position to the quencher and, therefore, an increase in amplification cycles leads to increase in fluorescence. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors. Advantages of this system include: more consistent Auto/ Y ratio; assessment of DNA degradation; reliable sample quality assessment; and flexible options for 4-point to 7-point standard curve. The PowerQuant System is optimized for use on the Applied Biosystems 7500 real-time PCR systems with v2.0.6 or HID1.1 or higher software versions.|
Use Robust, Instrument-Native Software for Most Analyses: Probe-based chemistry.
Reliably Quantify Sample: More consistent Auto/Y ratio.
Assess Sample Quality: Degradation marker included.
Achieve Sensitivity: Consistent and reproducible detection of 2pg of DNA.
Analyze Normalization for STR Analysis: Data analysis tool.
Process More Samples Per Plate: 4-point standard curve.