The Nano-Glo Live Cell Assay System is a single-addition, non-lytic detection reagent used to measure NanoBiT or NanoLuc luminescence from living cells. The reagent is prepared by diluting the Nano-Glo Live Cell Substrate with the Nano-Glo LCS Dilution Buffer to make the Nano-Glo Live Cell Reagent, a 5X stock that is added directly to cell culture medium. Both substrate and buffer solutions are optimized to provide enhanced stability. The Nano-Glo Live Cell Reagent is designed to reduce autoluminescence in the presence or absence of serum, increasing the sensitivity for detection of low levels of NanoBiT or NanoLuc luminescence. The Nano-Glo Live Cell Assay System can be used to monitor luminescence at a user-defined time point or continuously for up to 2 hours without compromising cell viability. Learn more about NanoLuc(R) technology at: www.promega.com/products/reporter-assays-and-transfection/reporter-assays/nanoluc-luciferase-redefining-reporter-assays/. Learn more about NanoBiT™ technology at: www.promega.com/products/protein-purification-and-interactions/protein-interactions/nanobit-complementation-assay-for-protein-interactions/|