NanoBRET Signaling Protein Assays are sensitive, reproducible live cell assays designed for monitoring or screening the interaction of proteins involved in cell signaling events. Interactions between proteins are key events in normal cellular signal transduction pathways, and modulation of these interactions has been implicated in disease formation, making them important candidates for drug targeting. The NanoBRET KRas/BRaf Interaction Assay measures the specific interaction between mutant KRas (G12C) and BRaf human proteins in their natural cellular context. In epidermal growth factor receptor (EGFR) pathway-associated oncogenesis, mutations in KRas result in constitutive binding to BRaf even in the absence of growth factor, resulting in cell proliferation and suppressed apoptosis. NanoBRET assay technology is dependent upon energy transfer from a luminescent donor (NanoLuc luciferase) to a fluorescent acceptor (HaloTag NanoBRET 618 Ligand). NanoLuc luciferase and HaloTag protein are fused to the target proteins of interest and fusion proteins expressed at low cellular levels, enabling monitoring and screening studies of protein interactions that reflect true cellular physiology. The NanoBRET assay is fully reversible, enabling studies of both induction and inhibition of protein interactions. For more details on NanoBRET technology and a full list of available NanoBRET Signaling Protein Interaction Assays visit the NanoBRET Technology for Protein Interactions page.|
Understand Real Biology: Live-cell assay allows you to measure signaling protein interactions in natural cellular context using full-length proteins expressed at low cellular levels.
Monitor Changes: Reversible assay technology allows you to study both induction and inhibition of protein interactions.
See Improved Assay Performance: Bright, blue-shifted donor signal and red-shifted acceptor create optimal spectral overlap, increased signal and lower background compared to conventional BRET assays.
Scale Your Assays: Assays can be performed in 96- or 384-well formats with low variability and high reproducibility, ideal for screening applications.
Enjoy Convenience: Fully optimized assays provide a sensitive and specific method to detect interactions of signaling protein targets; proven performance on GloMax(R), Discover System.