The Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System is a homogeneous reagent system that enables you to sequentially detect the activities of firefly (Photinus pyralis) luciferase and NanoLuc luciferase (Nluc) from a single sample. The firefly luciferase (Fluc) activity is measured first using ONE-Glo EX Luciferase Assay Reagent. NanoDLR Stop & Glo Reagent is added to quench the firefly signal and provide the furimazine substrate needed to measure Nluc activity. This convenient “add-read-add-read” format generates stable glow-type luminescent signals for both reporters directly from cells with no lysis or cell preconditioning steps required. Potent Fluc inhibition coupled with the high-intensity Nluc luminescence create a dual assay in which both reporters have maximum sensitivity in an assay format that is both easy-to-use and flexible. The NanoDLR workflow is compatible with assays or screens in any plate size, supports batch processing, and is ideal for any luminometer with no specific filter or injector requirements. Excellent signal separation allows for use of either Nluc, Fluc or both as the dynamic experimental reporter. Co-reporter control vectors expressing either Nluc or Fluc from a variety of promoters are available individually or can be obtained in reagent/vector bundles that provide the NanoDLR reagent with the TK or PGK control vector of choice for simple adoption of the NanoDLR Assay.|
Experience Improved Assay Performance: Better quenching of the Fluc signal and the bright Nluc co-reporter in a homogeneous assay format with stable signal kinetics for convenient “add-read-add-read” processing.
Achieve Greater Sensitivity: An Nluc signal up to 1 000 times brighter than Renilla luciferase and efficient separation of the Nluc and Fluc signals allow greater sensitivity, improved signal:background ratios and two independent reporters at full dynamic range.
Choose Your Assay Configuration: Use either Fluc or Nluc as the experimental reporter with the other as an internal normalization control, or multiplex with two experimental reporters for maximum data or expanded applications.
Notice Improved Ease-of-Use: Optimized reagents have greater stability, reducing requirements to aliquot and freeze, offer reduced reagent odor, and demonstrate decreased sensitivity to culture components.
Take Advantage of Workflow Flexibility: Designed as an “add-read-add-read” assay that can be used directly on cells; also compatible with injection-based protocols and cell lysates allowing use with any plate size up to 1,536-well format with minimal instrument limitations.