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cAMP-Glo Max Assay, 2 plates
Promega

cAMP-Glo Max Assay, 2 plates

Varenummer: V1681
Kort informasjon 2 plates
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The cAMP-Glo Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate GPCRs coupled with adenylate cyclase typically alter intracellular cAMP levels. The cAMP-Glo Max Assay monitors cAMP levels in cells in response to the effect of agonists, antagonists or test compounds on G protein-coupled receptors (GPCRs). The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction. This improved version combines the lysis and cAMP reaction buffers into the cAMP-Glo ONE Buffer. This new format streamlines the protocol and reduces the time needed to complete the assay. The new ONE Buffer is supplied at a 5X concentration, which provides increased flexibility for starting cell culture volumes. The cAMP-Glo Max Assay can be performed in 96-, 384- or 1536-well plates. The cells are induced with a test compound for an appropriate period of time to modulate cAMP levels. After induction, cells are lysed, and the cAMP released stimulates protein kinase A in the reagent (Figure 1). The Kinase-Glo Reagent is then added to terminate the PKA reaction and detect the remaining ATP via a luciferase reaction. Plates are read using a microplate-reading luminometer. The half-life for the luminescent signal is greater than 4 hours providing ample time to read the plates and eliminating the need for luminometers with reagent injectors.|

Fast and Easy to Use:
Improved-Lysis and cAMP detection steps combined (cAMP-Glo™ ONE Buffer).
ONE Buffer-5X concentration provides better flexibility for starting cell culture volumes.
Assay can be completed in approximately 30 minutes.
Excellent Signal-to-Noise Ratios:
Best signal:background ratio of all the cAMP assays.
Signal:Background more than200 (with cAMP), more than15 (on cells).
Easily scalable to 1536-well plate formats and beyond.
Proven Luminescent Technology:
Powered by Ultra-Glo™ Recombinant Luciferase.
No interference by fluorescent compounds.
Non-radioactive.

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Ekstra spesifikasjoner
Store the system at -20°C. Before use, completely thaw all components at room temperature, except for the Protein Kinase A, which should be kept on ice when not at -20°C. After thawing, mix all components thoroughly before use. Once prepared, the cAMP detection solution (cAMP-Glo™ ONE Buffer with Protein Kinase A) should not be frozen. Once prepared, the Kinase-Glo(R) Reagent should be dispensed into aliquots and stored at -20°C. See the product label for the expiration date.|Patent Pending.U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending. U.S. Pat. No. 7,700,310 and other patents and patents pending.U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.U.S. Pat. No. 7,741,067 and other patents and patents pending.The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

Kontaktperson(er) til dette produktet

Christine Rindal Ibra 944 34 009 christine.rindal.ibra@nmas.no
Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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