Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg(2+) or Mn(2+). The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.|
High Concentration: Cat.# M9004 contains 600 units of AMV RT at 20-25u/ ml.
5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25 C), 250mM KCl, 50mM MgCl2, 2.5mM spermidine, 50mM DTT.
Temperature Stability: AMV RT is the preferred reverse transcriptase for templates with high secondary structure due to its stability at higher reaction temperatures (37-58 C).