Promega

L-Rhamnose Monohydrate, 50 g

Varenummer: L5702
Kort informasjon 50 g
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The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression. These cells consolidate the best attributes of these two steps into one strain to evaluate protein expression in E. coli. Transformation efficiencies are greater than 10(8) cfu/ug, similar to other highly competent cells. The single step cells are available in both single (50ul) and multiple transformation (200ul) sizes. KRX can also be used for blue/white screening. Single Step (KRX) is an E. coli K strain that contains a chromosomal copy of the T7 RNA polymerase driven by a rhamnose promoter (rhaBAD) to provide dramatic control of the proteins expressed via a T7 promoter. Pre-induced expression protein levels are significantly lower than those of BL21(DE3)-derived strains. This feature facilitates cloning and expression of proteins toxic to E. coli. Genotype: [F', traD36, DeltaompP, proA+B+, lacIq, Delta(lacZ)M15] DeltaompT, endA1, recA1, gyrA96 (Nal(r)), thi-1, hsdR17 (r(k)-, m(k)+), e14- (McrA-), relA1, supE44, Delta(lac-proAB), Delta(rhaBAD)::T7 RNA polymerase.|
Save Time: In two days, you can transform your vector into the Single Step (KRX) Competent Cells and be ready for protein expression.Controlled Protein Expression: For overall expression of cloned proteins, the Single Step (KRX) Competent Cells provide dramatic control of expressed protein-coding regions.Achieve High Yields: Protein expression levels were shown to be as high as or higher than levels expressed in BL21(DE3)-derived strains.Blue/White Screening: Convenient method for detecting recombinant clones.

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Always store competent cells at -70 C. Thaw on ice when ready for use. Do not refreeze thawed, unused aliquots.|Patent Pending. Usage restrictions apply to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof. Usage Restrictions for the T7 Expression SystemThe T7 expression system is based on technology developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy and is the subject of patents assigned to Brookhaven Science Associates, LLC (BSA). This technology, including bacteria, phage and plasmids that carry the gene for T7 RNA polymerase, is to be used for academic or nonprofit laboratory or licensed commercial research purposes only. By accepting or using the T7 expression technology you agree to be bound by the following conditions set forth by BSA. The initial purchaser may refuse to accept the conditions of this notice by returning this product and the enclosed materials to Promega unused. Academic and NonProfit LaboratoriesNo materials that contain the cloned gene for T7 RNA polymerase may be distributed further to third parties outside of your laboratory unless the recipient receives a copy of this assurance notice and agrees to be bound by its terms. This limitation applies to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof. Commercial LaboratoriesA license is required for any commercial use of the T7 expression system, including use of the T7 system for research purposes or for production purposes by any commercial entity. Information about commercial licenses may be obtained from the Licensing Office, Brookhaven National Laboratory, Upton, NY 11973, Telephone: 631-344-7134, FAX: 631-344-3729.

Kontaktperson(er) til dette produktet

Christine Rindal Ibra 944 34 009 christine.rindal.ibra@nmas.no
Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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