SoftLink Avidin Resin can be used for the isolation and purification of biotinylated molecules. SoftLink Resin is a rigid, methacrylate polymeric gel filtration matrix, functionalized with covalently bound, monomeric avidin. Monomeric avidin binds biotin with a K(d) value of 10(-7)M, allowing reversible binding of bound biotinylated proteins under mild elution conditions. Native, or tetrameric, avidin binds biotin with a very strong affinity (K(d) = 10(-15)M), which in turn requires strong denaturing conditions for eluting bound material. Monomeric avidin allows the specificity of capture but also the mildness of release appropriate for the purification of sensitive biological materials.|
Sensitive: Binds 20-40nmol of biotinylated protein per milliliter of resin. Easy to Use: Bound biotinylated molecules can be eluted under mild nondenaturing conditions (5mM biotin). Versatile: Retains biotin binding ability following exposure to a wide range of pH, low or high ionic strength, 6M guanidine and 1% SDS. Reusable: Regenerates at least 10 times without loss of binding capacity. Robust: Supports high flow rates (300cm/hour) and centrifugal forces (1,500 x g) in batch applications. Flexible: Purifications by batch or column method.