The HaloTag Mammalian Protein Purification System (Cat.# G6790) is an optimized kit for purification of HaloTag fusion proteins from mammalian cell culture lysates. HaloTag is a protein fusion tag engineered to form a highly specific and covalent bond with the HaloLink Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag Mammalian Protein Detection and Purification System (Cat.# G6795) also includes HaloTag TMRDirect Ligand. The simple-to-use fluorescent detection of the HaloTag fusion facilitates rapid optimization of expression and purification conditions.|
Purify More Protein: HaloLink™ Resin covalently binds more than7mg/ml of HaloTag(R) fusion protein (10X more capacity compared to FLAG(R)). Recovery is highly efficient, commonly more than75%.Higher Purity: Covalent capture allows extensive and/or stringent washes without loss of bound protein, resulting in very low (less than0.1%) nonspecific binding and a highly pure protein.Easily Scalable: Scale up and down, important for obtaining mg-plus quantities.Optimized for Mammalian Protein Expression: The HaloTag(R) platform allows flexibility to move between purification, pull-downs and cellular imaging with a single construct.