Six DNA Fragments of 3,530–21,226bp
The Conventional DNA Digest Markers are created by digesting either lambda DNA, PhiX174 replicative form DNA, or plasmids to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. The markers are not intended for quantitative analysis. Each marker is supplied with a tube of 6X Blue/Orange Loading Dye. Lambda DNA/HindIII Markers: Eight ethanol-precipitated DNA fragments ranging in size from 125bp to 23,130bp. Lambda DNA/EcoRI Markers: Six ethanol-precipitated DNA fragments ranging in size from 3,530bp to 21,226bp. Lambda DNA/EcoRI + HindIII Markers: Thirteen ethanol-precipitated DNA fragments ranging in size from 125bp to 21,226bp. PhiX174 DNA/HaeIII Markers: Eleven phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 72bp to 1,353bp. PhiX174 DNA/HinfI Markers: Twenty ethanol-precipitated DNA fragments ranging in size from 24bp to 726bp. pGEM DNA Markers: Fifteen phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 36bp to 2,645bp. These unique markers are generated from separate digests of pGEM-3 Vector DNA with HinfI, RsaI and AvaII later combined to form the markers.|