Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors). Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cell debris. The supernatant is subjected to chromatography that separates endogenous amino acids and plant pigments from the extract. The extract also is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum.|
Optimized: Extract contains an energy-regenerating system (phosphocreatine/phosphocreatine kinase), spermidine (to stimulate the efficiency of chain elongation), magnesium acetate and potassium acetate.
Flexible: Three Amino Acid Mixtures are provided, which enable different choices of radioisotopes.
Robust: Potassium Acetate is provided to enhance translation for a wide range of mRNAs.
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