The pGEM-9Zf(-) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, efficient synthesis of RNA in vitro and the production of single-stranded DNA. The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for NsiI, SpeI, HindIII, XbaI, EcoRI, SalI and SacI.|
Excisable SP6/T7 Insert: This vector allows the excision of an insert containing the SP6 and T7 RNA polymerase promoters.Blue/White Screening: Allows the easy identification of recombinant clones.Versatile: This vector can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.Convenient: Multiple cloning site provides a selection of restriction sites for cloning.