The MagneGST Protein Purification System provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins. Immobilized glutathione paramagnetic particles (MagneGST Particles) are used to isolate GST-tagged protein directly from a crude or cleared lysate using either a manual or automated procedure and requires use of a magnetic stand. GST-tagged proteins can be purified on a small scale from 1ml of culture or on a large scale using more than 50ml of culture. Samples also can be processed using a robotic platform. MagneGST particles are supplied as a 25% slurry and have a binding capacity of 5-10mg of GST protein per 1ml of settled resin.|
Simple: One-step purification of multiple samples with easy handling. Quick: After cell lysis, no requirement for high-speed centrifugation to clear lysate. Scalable: Scalable protocol using 1-50ml of cell culture. Efficient: Achieve high yields with little or no nonspecific background.