ProteaseMAX Surfactant, Trypsin Enhancer, is designed to improve in-gel and in-solution protein digestion. ProteaseMAX Surfactant ensures fast and efficient protein digestion with proteases such as Trypsin, Chymotrypsin and Lys-C. For in-gel protein digestion, ProteaseMAX Surfactant offers time and labor savings. Digestion step is complete in 1 hour, and the surfactant provides concurrent extraction of peptides from gels, eliminating the need for post-digestion peptide extraction. The surfactant also improves recovery of longer peptides that are retained in the gel under a standard extraction protocol. For additional data, refer to scientific posters PS094 and PS099 (Product Resources tab). For in-solution digestions, ProteaseMAX Surfactant solubilizes proteins, including difficult proteins (i.e., membrane proteins), and enhances protein digestion by providing a denaturing environment prior to protease addition. ProteaseMAX Surfactant degrades over the course of a digestion reaction, yielding products that are compatible with downstream methods such as mass spectrometry (MS) and liquid chromatography (LC). No long-term negative effect of the residual surfactant on the ion optics and capillary of mass spectrometers has been observed. ProteaseMAX Surfactant can be used with existing in-gel or in-solution digestion protocols.|
No Peptide Extraction Required Following In-Gel Digestions: Save time and increase the number of samples processed. Improved Peptide Recovery from Gels: Increase protein sequence coverage, thus increasing confidence of protein identification (Figure 1). Enhanced Protein Solubilization: Solubilize complex proteins, such as membrane proteins, at room temperature, avoiding high temperature and preventing precipitation (Figure 2). Degrades Over Course of Digestion: Samples are ready for use directly for mass spectrometry analysis without additional inactivation steps such as heating or acid treatment.