Glo Lysis Buffer (GLB), 1X, is a proprietary formulation developed to promote rapid lysis (within 5 minutes) of cultured mammalian cells without scraping or performing freeze-thaw cycles. It is fully compatible with Bright-Glo, Steady-Glo, ONE-Glo and Renilla-Glo Luciferase Assay Reagents and the Luciferase Assay Reagent for analysis of firefly luciferase expression. The half-life of these reagents remains the same with or without use of GLB, more than5 hours for Steady-Glo Reagent and more than24 minutes for Bright-Glo Reagent.|
Convenient: No need for cell scraping or freeze-thaw cycles.Fast: Cell lysis within 5 minutes.Versatile: Use with Bright-Glo(TM);, Steady-Glo(R),, ONE-Glo(TM); and Renilla-Glo(R), Luciferase Assay Reagents to provide nonhomogeneous assay formats or with other reporter applications.Robust: Firefly luciferase enzyme in Glo Lysis Buffer is stable at room temperature for at least 48 hours.