High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo Luciferase Assay System is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample processing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.|
Greater Light Output: Greater assay sensitivity than other extended-lifetime firefly luciferase assay reagents.
Improved Assay Precision and Reproducibility: Less sensitive to mixing conditions in multiwell plates. Particularly useful in 384-well plates.
Convenience: Simply mix buffer with lyophilized substrate and add to cells in culture medium; no need to thaw or measure before use.
No Sample Preprocessing: No need to remove culture medium or wash cells prior to adding assay reagent. Grow cells and assay them directly within the same multiwell plate.
Easy to Use: Simply add reagent, which contains a cell lysis component, wait 5 minutes and measure luminescence.
Robust: Compatible with many tissue culture media, including those containing up to 10% serum.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/