The CellTiter-Glo One Solution Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which indicates the presence of metabolically active cells. This frozen, ready-to-use format is based on the original CellTiter-Glo Luminescence Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when preparing reagent. The CellTiter-Glo Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) in 96- to 1536-well format, and cell proliferation and cytotoxicity assays. The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo One Solution Assay generates a stable "glow-type" luminescent signal with a half-life of greater than three hours. This extended half-life eliminates the need for reagent injectors and provides flexibility for continuous or batch-mode processing of multiple plates.|
Convenient: No reagent preparation is required; simply thaw and "add-mix-measure". Volumes convenient for HTS applications.Homogeneous: "Add-mix-measure" format reduces the number of plate-handling steps.Fast: Data can be recorded 10 minutes after reagent addition. Sensitive: Measures cells at numbers below the detection limits of standard colorimetric and fluorometric assays.Flexible: Can be used with various multiwell formats (96-, regular or low-volume 384- and 1536-well plates). Data can be recorded by luminometer or CCD camera imaging device.Robust: Stable luminescent signal with a half-life more than3 hours, depending on cell type and culture medium used.Ability to Multiplex: Can be used with other nonlytic compatible assay chemistries from Promega.