The ONE-Glo Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high- and ultrahigh-throughput applications, the ONE-Glo Assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor than standard luciferase assay reagents. These features ensure that the ONE-Glo Assay provides robust performance and also eliminates many of the handling inconveniences experienced using other reporter assays in a high-throughput setting.|
Simplified Assay Optimization: Robust performance, reduced odor, improved storage and larger available sizes. Room Temperature or 4 C Storage: Extended stability of the ONE-Glo(TM); Reagent makes it more convenient for everyday use. Improved Assay Precision: The ONE-Glo(TM); Reagent is less sensitive to mixing and dispensing conditions, enhancing reproducibility. Ideal for use in high-density (384- and 1536-well) microplates. Brighter, Longer-Lasting Signal: Optimized for batch and continuous-process handling, the extended bright light output allows high sensitivity, especially for extended incubations. Reduced Unwanted Effects from Sample Components: The ONE-Glo(TM); Assay is less sensitive to culture media, phenol red and luciferase inhibitors than other luciferase assays.