The pGL2 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The pGL2 Vectors carry the coding region for firefly (Photinus pyralis) luciferase, which is used to monitor transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the pGL2 Vectors contain numerous features that aid in the characterization and mutagenesis of the putative regulatory sequences.|
Versatile: Deletions and site-directed mutations can be made directly to inserted DNAs without subcloning.Convenient: All vectors contain the firefly luciferase reporter gene, which enables sensitive and rapid quantitation of reporter activity.Low Background: Upstream polyadenylation signal minimizes spurious transcription of the reporter gene.