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MagneGST Pull-Down System
Promega

MagneGST Pull-Down System

Varenummer: V8870
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  • Spesifikasjoner
The MagneGST Pull-Down System is designed for detection of protein interactions between GST-fusion proteins expressed in bacterial lysates and prey proteins expressed in the TNT Systems. Prey protein synthesized in the TNT Quick Coupled Transcription/Translation Reaction is captured using bait protein (GST-fusion protein) immobilized on MagneGST Particles. Nonspecifically bound proteins are then washed away, and the prey protein is analyzed. Prey proteins can be detected by incorporating radioactively labeled methionine in the TNT Quick reaction, followed by SDS-PAGE and autoradiography or by incorporating the supplied non-radioactive methionine in the TNT reaction and detecting by Western blotting with protein-specific antibodies.|

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Ekstra spesifikasjoner
Store the TnT(R) T7 Quick Master Mix and Methionine at -70°C. Store the RQ1 RNase-Free DNase at -20°C. Store the Nuclease-Free Water, MagneGST™ Glutathione Particles, MagneGST™ Binding/Wash Buffer and Cell Lysis Reagent at 4°C.|U.S. Pat. No. 7,319,021 and other patents pending.U.S. Pat. Nos. 5,324,637 and 5,492,817 and other patents.U.S. Pat. No. 5,552,302.U.S. Pat. No. 6,242,235, Australian Pat. No. 769757, Canadian Pat. No. 2,335,153 and other patents and patents pending.This product is licensed for use under U.S. Pat. No. 6,174,704.Any use of the product for diagnostics requiring clearance or approval by the U.S. Food and Drug Administration or a foreign equivalent may require a license under Mayo Clinic U.S. Pat. No. 6,027,913.Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Kontaktperson(er) til dette produktet

Christine Rindal Ibra 944 34 009 christine.rindal.ibra@nmas.no
Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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