The phMGFP Vector contains the open reading frame for the Monster Green Fluorescent Protein cloned into a mammalian expression vector. The Monster Green Fluorescent Protein is encoded by an improved synthetic version of the green fluorescent protein gene originally cloned from Montastrea cavernosa (Great Star Coral). The synthetic gene (hMGFP) expresses a 26kDa protein that shows improved fluorescence intensity compared to the native gene. Furthermore, the hMGFP gene has been codon optimized and cleared of most consensus sequence transcription factor binding sites to ensure reliability and high levels of expression. The Monster Green Fluorescent Protein encoded by the hMGFP gene is an ideal fluorescent reporter, providing high-level fluorescence and reducing cytotoxicity. Monster Green Fluorescent Protein generally fluoresces at least 20% brighter than other commercially available green fluorescent proteins (GFPs) and also reduces cytotoxicity, offering flexibility when working with transient and stable expression assays.|
Brighter Fluorescence: Visualize low-level expression in situ using fluorescence microscopy, imagers or FACS(R).Reduced Cytotoxicity: Minimize cellular perturbations when working with transient or stable expression assays.Flexible: Create fusion proteins for imaging and localization studies using standard FITC detection.High Purity: Obtain high transfection efficiencies for precloning confirmation studies.