The MSI Analysis System, Version 1.2, is a fluorescent multiplex PCR-based method for detection of microsatellite instability (MSI), a form of genomic instability. This instability is due to either insertion or deletion of repeating units during DNA replication and failure of the mismatch repair system (MMR) to correct these errors. MSI analysis typically involves comparison of allelic profiles of microsatellite markers generated by amplification from matching pairs of test samples, which may be MMR-deficient, and normal tissue samples. New alleles in the abnormal sample not found in the corresponding normal sample indicate the presence of MSI. MSI analysis can be used as a screening method to identify samples for further characterization. The MSI Analysis System, Version 1.2, includes fluorescently labeled primers (marker panel) for co-amplification of seven markers for analysis of the MSI-high (MSI-H) phenotype, including five nearly monomorphic mononucleotide repeat markers (BAT-25, BAT-26, MON0-27, NR-21 and NR-24) and two highly polymorphic pentanucleotide repeat markers (Penta C and Penta D). Amplified fragments are detected using an ABI PRISM 310, 3100, 3100-Avant, 3130 or 3130xl Genetic Analyzer after spectral calibration. Panels and bins text files simplify and standardize data analysis by providing automated assignment of genotypes using GeneMapper 4.0 software.|
Understand the Complete MSI Phenotype: A single multiplex PCR amplifies five informative mononucleotide repeat markers for MSI-H determination.Confidence in Sample Identification: Co-amplification of highly polymorphic pentanucleotide repeats provides internal sample tracking.Consistent Data Analysis: MSI Panels and bins for GeneMapper(R), software can be downloaded.