TSAP Thermosensitive Alkaline Phosphatase catalyzes the removal of 5' phosphate groups from DNA, thus preventing the recircularization and religation of linearized cloning vector DNA during ligation. It is effective on 3' overhangs, 5' overhangs and blunt ends. It is also useful for preparing DNA for 5' end-labeling by removing existing phosphate groups from the 5' end. TSAP is irreversibly inactivated by heating at 74 degrees C for 15 minutes. Therefore, a DNA cleanup step is not required before proceeding to a ligation reaction. TSAP is fully active in all restriction enzyme reaction buffers tested under the conditions listed below, facilitating a streamlined restriction digestion, dephosphorylation and ligation reaction.|
Easy To Use: TSAP is active in all Promega restriction enzyme buffers, eliminating any cleanup steps or buffer swaps.Convenient: TSAP is irreversibly inactivated by heating at 74°C for 15 minutes. This allows streamlining of the restriction enzyme digestion, dephosphorylation and ligation procedure by eliminating the need for cleanup after alkaline phosphatase treatment.Blue/White Cloning-Qualified: Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.Provided with Promega MULTI-CORE™ Buffer.