Alkaline Phosphatase, Calf Intestinal (CIAP), catalyzes the hydrolysis of 5'-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vector DNA by removing phosphate groups from both 5'-termini and may also be used for the dephosphorylation of 5' phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 Polynucleotide Kinase. CIAP is active on 5' overhangs, 5' recessed and blunt ends.|
Available at High Concentration: Cat.# M2825 contains 1 000 units of CIAP at 20u/ ml.
Blue/White Cloning Qualified: Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.
Provided with 10X Reaction Buffer: 0.5M Tris-HCl (pH 9.3 at 25 C), 10mM MgCl2, 1mM ZnCl2, 10mM spermidine.