DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is a DNA-dependent DNA polymerase that lacks both the 5'--more than3' and the 3'--more than5' exonuclease activities present in intact E. coli DNA Polymerase I. It is used for random primer labeling and in strand displacement amplification. Klenow Fragment, Exonuclease Minus, will leave a single-base 3' overhang on a significant proportion of DNA fragments during fill-in of 5'-overhangs. Therefore, this enzyme is not recommended for preparation of blunt-ended fragments for ligation.|
Provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT. May Be Heat-Inactivated: DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is inactivated by heating at 75°C for 10 minutes.