T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids.|
Available at High Concentration: Cat.# M1794 contains 500 units of T4 DNA Ligase at 10-20u/ ml.
Flexible: Use with 5´, 3´ or blunt-ended DNA inserts.
Provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25 C), 100mM MgCl2, 100mM DTT and 10mM ATP.
Blue/White Cloning Qualified: Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/