The FluoroTect Green(Lys) in vitro Translation Labeling System allows for the fluorescent labeling and detection of proteins synthesized in vitro. The system is based on a lysine-charged tRNA that is labeled at the epsilon position of the lysine with the fluorophore BODIPY-FL. Fluorescent lysine residues will be incorporated into synthesized proteins during in vitro translation reactions, eliminating the need for radioactivity. Detection of the labeled proteins is accomplished in 2-5 minutes directly in-gel by use of a laser-based fluorescent gel scanner. This eliminates any requirements for protein gel manipulation such as fixing/drying or any safety, regulatory and waste disposal issues associated with the use of radioactively labeled amino acids use. The convenience of in-gel detection also avoids the time-consuming electroblotting and detection steps of conventional non-isotopic systems.|
Fast: Data can be obtained in minutes, eliminating overnight exposures associated with radioactive-based systems or time-consuming steps utilized by traditional non-isotopic methodologies. Convenient: Results based on "in-gel" detection. No requirement to transfer, fix, or dry gels. Non-Radioactive: No safety, regulatory or waste disposal issues associated with radioactivity. Flexible: The modified charged tRNA can be used with a variety of Promega translation systems including: Rabbit Reticulocyte Lysate, TnT(R) Coupled Transcription/Translation System, Wheat Germ Extract and E. coli S30 Extract.