The Luciferase SP6 and T7 Control DNAs are used as functional controls in the TNT Quick Coupled and TNT Coupled Transcription/Translation Systems. The Control DNAs contain the gene for luciferase under transcriptional control of a phage RNA polymerase promoter. All constructs carry a 30-base pair poly[d(A)/d(T)] tail following the luciferase gene. Control reactions are monitored easily by the production of luminescence, which is generated from full-length luciferase and the addition of necessary components. Luciferase Control DNAs are supplied as 0.5mg/ml solutions in TE buffer.|