These vectors are designed for expression of C-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, the HaloTag fusion protein may be used for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands. In addition, the HaloTag fusion protein can be purified or pulled down as a complex with its protein partners. We offer two types of HaloTag fusion vectors to accommodate your cloning preferences: pHT Vector Series: Simple Multiple Cloning Site (MCS) plasmids for traditional cloning. pF Vector Series: Flexi Vector Cloning System---a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence. Note: Flexi Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert. Find your gene, precloned, and experimentally validated at: www.promega.com/products/pm/halotag-technology/kazusa-collection|
Versatility: You can choose between a variety of initial applications (e.g., bacterial protein, mammalian, or cell-free protein expression) and then transfer to others as required.Time Savings: Efficient transfer allows for direct use of recombinant clones, minimizing time wasted screening background colonies.Enhanced Productivity: Adaptable to high-throughput formats for large screening projects.Easy Access: No licensing fees or complicated transfer restrictions.