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HaloTag Complete Pull-Down System
Promega

HaloTag Complete Pull-Down System

Varenummer: G6509
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The HaloTag Mammalian Pull-Down Systems (Cat.# G6500, G6504 and G6509) are designed to capture and purify intracellular binary and higher order protein complexes, including transient or weakly interacting partners. HaloTag Mammalian Pull-Down System (Cat.# G6504) includes buffers and resin necessary to perform a HaloTag pull-down. HaloTag Mammalian Pull-Down and Labeling System (Cat.# G6500) includes everything in G6504 plus the HaloTag TMRDirect Ligand, which allows correlative cellular localization and real-time imaging studies. HaloTag Complete Pull-Down System (Cat.# G6509) includes everything in G6500 plus a starter cloning system, Wizard SV Gel and PCR Clean-Up System, and FuGENE HD Transfection Reagent. The HaloTag Control Vector provides protein expression of the HaloTag protein in mammalian cells, E. coli or in vitro expression systems dependent on human cytomegalovirus (CMV) intermediate early enhancer, T7 or SP6 RNA polymerase promoters. It can be used as a control for any HaloTag experimental system and can be used for both stable and transient HaloTag expression in mammalian cells; for stable expression, co-transfection with a vector containing a selectable marker is required. The Protease Inhibitor Cocktail, 50X, is a mixture of six different protease inhibitors with different target protease specificities. This product is provided in a freeze-dried format and can be reconstituted using either 100% ethanol or DMSO. The Mammalian Lysis Buffer is designed for use with HaloTag Mammalian-based expression systems such as the HaloTag Mammalian Pull-Down and Labeling Systems (Cat.# G6500 and G6504) as well as the HaloCHIP System (Cat.# G9410). Formulation consists of 50mM Tris-HCl, 150mM NaCl, 1% Triton X-100 and 0.1% sodium deoxycholate (pH 7.5).|
Rapid, Efficient and Covalent Capture of Binary and Higher Order Complexes Directly from Lysates: Improved capture of protein partners, including transient interactions.High Purity and Low Background: Improved accuracy in identification of proteins; covalent attachment allows bait protein to remain behind if desired.Ability to Fluorescently Label the Same Genetic Fusion: Correlate complex capture with cellular localization.Compatibility with All Downstream Methods of Analysis: Freedom to identify complexes in variety of applications including mass spectrometry.

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Ekstra spesifikasjoner
Store the 10X TBS Buffer and SDS Elution Buffer at room temperature. Store the HaloLink(TM); Resin and Mammalian Lysis Buffer at 4 C. Store the HaloTag(R), TMRDirect(TM); Ligand and powdered Protease Inhibitor Cocktail at -30 to -10 C. Reconstituted Protease Inhibitor Cocktail can be stored at 2-10 C for 12 months.Cat.# G6509, G6500, G6504: BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.Researchers may only use this product in their own research and may modify or link Promega HaloTag(R), ligands to Promega or customer-supplied moieties. No other use or transfer of this product is authorized without the express, written consent of Promega including, without limitation, commercial use. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.Cat.# G6509, G6591: BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag(R), gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag(R), gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag(R), gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag(R), gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag(R), ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag(R), ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.Cat.# G6509, G6591: U.S. Pat. Nos. 7,425,436 and 7,935,803 and other patents pending.Cat.# G6509, G6591: For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.Cat.# G6509: U.S. Pat. Nos. 7,429,472, 7,888,086, 7,867,726 and other patents pending.Cat.# G6509: Patent Pending.Cat.# G6509: European Pat. No. 1685247 and other patents pending.Cat.# G6509: FuGENE(R), HD is sold with a Limited Use Label License (LULL).You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

Kontaktperson(er) til dette produktet

Christine Rindal Ibra 944 34 009 christine.rindal.ibra@nmas.no
Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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