The BenchTop DNA Markers offer the convenience of storage at room temperature (22-25°C) as well as the capability of direct loading onto agarose gels. The BenchTop DNA Markers are supplied in a stabilizing solution of 1X Blue/Orange Loading Dye, which circumvents any requirements for further manipulation. BenchTop ΦX174 DNA/HaeIII Markers: Eleven phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 72bp to 1,353bp. BenchTop pGEM(R) DNA Markers: Fifteen phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 36bp to 2,645bp. These unique markers are generated from separate digests of pGEM(R)-3 Vector DNA with HinfI, RsaI and SinI later combined to form the markers. BenchTop PCR Markers: Six bands of equal intensity of 50, 150, 300, 500, 750, and 1,000bp. The BenchTop PCR Markers may be run on polyacrylamide gels with less loading volume; however, additional bands may be visible compared to those visible on agarose gels. BenchTop 1kb DNA Ladder: Thirteen blunt-ended fragments with sizes ranging from 250bp to 10,000bp. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromide-stained agarose gels for easy identification. All other fragments are of equal intensity. The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4 Polynucleotide Kinase, allowing visualization by autoradiography. BenchTop 100bp DNA Ladder: Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. The 500bp fragment is present at increased intensity for easy identification. The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4 Polynucleotide Kinase, allowing visualization by autoradiography.|
Convenient: Storage at 22-25°C.Efficient: Premixed with loading buffer. Ready to load onto agarose gels.Versatile: Five different BenchTop DNA Markers available.