The Conventional DNA Digest Markers are created by digesting either lambda DNA, PhiX174 replicative form DNA, or plasmids to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. The markers are not intended for quantitative analysis. Each marker is supplied with a tube of 6X Blue/Orange Loading Dye. Lambda DNA/HindIII Markers: Eight ethanol-precipitated DNA fragments ranging in size from 125bp to 23,130bp. Lambda DNA/EcoRI Markers: Six ethanol-precipitated DNA fragments ranging in size from 3,530bp to 21,226bp. Lambda DNA/EcoRI + HindIII Markers: Thirteen ethanol-precipitated DNA fragments ranging in size from 125bp to 21,226bp. PhiX174 DNA/HaeIII Markers: Eleven phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 72bp to 1,353bp. PhiX174 DNA/HinfI Markers: Twenty ethanol-precipitated DNA fragments ranging in size from 24bp to 726bp. pGEM DNA Markers: Fifteen phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 36bp to 2,645bp. These unique markers are generated from separate digests of pGEM-3 Vector DNA with HinfI, RsaI and AvaII later combined to form the markers.|