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GoTaq® Probe qPCR Master Mix, 2 ml
Promega

GoTaq® Probe qPCR Master Mix, 2 ml

Varenummer: A6101
Kort informasjon 200 × 20 µl reactions The GoTaq® Probe qPCR Master Mix is a ready-to-use, stabilized, 2X formulation with all of the components for quantitative PCR (qPCR).
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The GoTaq Probe qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format. The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. This master mix does not contain a reference dye; a separate tube of CXR reference dye is included. The GoTaq Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript Reverse Transcription System and GoTaq Probe qPCR Master Mix. The GoScript Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions. The GoTaq Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript Reverse Transcriptase and GoTaq Probe qPCR Master Mix in a single-step real-time amplification reaction. The GoScript RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript Reverse Transcriptase, RNasin Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.|

Achieve Superior Performance: Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
Rely on a Robust System: Resistant to a wide range of PCR inhibitors.
Use a Versatile Product: Compatible with both fast and standard cycling methods on most real-time PCR instruments using TaqMan(R), and other probe assays such as molecular beacons.
Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated and high-throughput detection.

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Ekstra spesifikasjoner
Store all components between -30 C and -10 C. Protect components from light at all times. For best results, mix thawed solutions gently to minimize aeration and foaming, and keep on ice. For short-term storage and frequent use, the GoTaq; qPCR Master Mix, 2X, may be kept at 2-8 C for up to 3 months if protected from light.Cat.# A6110: U.S. Pat. No. 5,552,302.All Cat.# on this page: U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.All Cat.# on this page: Licensed under U.S. Pat. No. 5,587,287 and corresponding patents in other countries. For Research Use Only. Not for use in diagnostic procedures.All Cat.# on this page:NOTICE TO PURCHASER: DISCLAIMER OF LICENSENo license is conveyed with the purchase of this product under any of US Pat. Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787 and 6,258,569 and corresponding patents outside the United States or any other patents or patent applications relating to the 5´ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 USA.

Kontaktperson(er) til dette produktet

Christine Rindal Ibra 944 34 009 christine.rindal.ibra@nmas.no
Claudia Emmanuel 951 51 950 claudia.emmanuel@nmas.no
Monica Laukas 404 40 960 monica.laukas@nmas.no

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