Pfu DNA Polymerase is a thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75 degrees C, catalyzing the polymerization of nucleotides into duplex DNA in the 5'--more than3' direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3'--more than5' exonuclease (proofreading) activity. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Consequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended. Pfu DNA Polymerase 10X Reaction Buffer with MgSO4: 200mM Tris-HCl (pH 8.8 at 25 degrees C), 100mM KCl, 100mM (NH4)(2)SO4, 20mM MgSO4, 1.0% Triton X-100 and 1mg/ml nuclease-free BSA.|
High Fidelity: Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase.Complete: Provided with 10X buffer containing 20mM MgSO4.Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.