DNA Polymerase I Large (Klenow) Fragment is a DNA-dependent DNA polymerase that lacks the 5'--more than3' exonuclease activity of intact E. coli DNA Polymerase I but retains its 5'--more than3' polymerase, 3'--more than5' exonuclease and strand displacement activities. The enzyme is a 68kDa C-terminal fragment of DNA Polymerase I. The 5'--more than3' polymerase activity of Klenow Fragment can be used to fill in 5'-protruding ends with unlabeled or labeled dNTPs, to sequence single- or double-stranded DNA templates, for in vitro mutagenesis using synthetic oligonucleotides, for cDNA second-strand synthesis and to generate single-stranded DNA probes. The 3'--more than5' exonuclease activity can be used to generate blunt ends from a 3'-overhang.|
Flexible: DNA Polymerase I Large (Klenow) Fragment may be used in a variety of molecular applications. It is also active in many Promega 1X restriction enzyme buffers.
May Be Heat-Inactivated: DNA Polymerase I Large (Klenow) Fragment is inactivated by heating at 75 C for 10 minutes.
Provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.2 at 25 C), 100mM MgSO4, 1mM DTT.
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