Terminal Deoxynucleotidyl Transferase, Recombinant, catalyzes the repetitive addition of mononucleotides to the terminal 3'-OH of a DNA initiator accompanied by the release of inorganic phosphate. Single-stranded DNA is preferred as an initiator. Polymerization is not template-dependent. The addition of 1mM Co(2+) (as CoCl(2)) in the reaction buffer allows the tailing of 3'-ends with varying degrees of efficiency.|
Tails Any Type of 3´ End: The presence of 1mM CoCl2 in the reaction buffer allows the tailing of any type of 3´ end (3´ and 5´ overhangs or blunt ends).
Tested for Apoptotic DNA Labeling: Each lot of enzyme is qualified for success in the procedure outlined in the DeadEnd(TM); Fluorometric TUNEL System Technical Bulletin #TB235.
Provided with 5X Reaction Buffer: 500mM cacodylate buffer (pH 6.8 at 25 C), 5mM CoCl2, 0.5mM DTT.
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