ImProm-II Reverse Transcriptase enables robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The ImProm-II Reverse Transcriptase can be used to reverse transcribe total RNA, poly(A)+ mRNA or synthetic transcript RNA templates.|
Full-Length RT-PCR: Reverse transcribe RNA templates up to 8.9kb.
Microarray-Compatible: Incorporate regular and Cy(R),3- and Cy(R),5-modified nucleotides.
Scalable and Flexible: Reaction volumes of 1-20 ml can be used in subsequent PCR, and the optimized buffer also allows for coupled RT-PCR.
Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25 C), 375mM KCl and 50mM DTT. A 25mM MgCl2 Solution also is included.
Versatile: Use with your thermostable polymerase of choice for two-step RT-PCR.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/