pGEM-T Easy Vector System II

The pGEM-T Easy Vector Systems are convenient systems to clone PCR products. They offer all of the advantages of the pGEM-T Vector Systems with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM-T Easy Vector System II contains JM109 Competent Cells in addition to all of the pGEM-T Easy Vector System I components.|

Flexibility: The multiple cloning site is flanked by restriction enzyme sites for BstZI, NotI and EcoRI, giving you three options to remove the insert with a single digest.
Rapid Ligation: The provided 2X Rapid Ligation Buffer allows reactions to be completed in 1 hour at room temperature.
Blue/White Screening: T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by color screening on indicator plates.
f1 Origin of Replication: Allows preparation of single-stranded DNA.