The pGEM-5Zf(+) Vector is derived from the pGEM-3Zf(+) Vector and contains the origin of replication of the filamentous phage f1. This plasmid serves as a standard cloning vector, as a templates for in vitro transcription and can be used for the production of circular ssDNA. This vector contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base System.|
Blue/White Screening: Allows the easy identification of recombinant clones.Versatile: This vector can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.Convenient: Multiple cloning site provides a selection of restriction sites for cloning.Unidirectional Deletions: Restriction sites are positioned conveniently for use with the Erase-a-Base™ System.