T4 Polynucleotide Kinase catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group. The enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.|
May Be Heat-Inactivated: T4 Polynucleotide Kinase may be inactivated by heating at 68 C for 10 minutes.
Provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25 C), 100mM MgCl2, 50mM DTT.
Blue/White Cloning Qualified: Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/