Bacterial Strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase. JM109(DE3) is used for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, provided that the cloned sequence contains a ribosomal binding site. Please note: JM109(DE3) cannot be used for blue/white selection. Genotype: endA1, recA1, gyrA96, thi, hsdR17 (r(k)-, m(k)+), relA1, supE44, lambda-, Delta(lac-proAB), [F', traD36, proAB, lacI(q)ZDeltaM15], lDE3.|
T7 Promoter Expression: Contains an IPTG-inducible gene for T7 RNA polymerase.